rabbit anti mouse cd14 polyclonal antibodies  (Boster Bio)

Journal: Annals of Neurosciences

Article Title: Motor Recovery after Transplantation of Bone Marrow Mesenchymal Stem Cells in Rat Models of Spinal Cord Injury

doi: 10.1159/000487069

Figure Lengend Snippet: Immunohistochemical characterization of rat MSC. Rat MSCs express positivity for the panel of markers CD29, CD54, CD90, CD73, and CD105 and negativity for hematopoietic markers CD34, CD14, and CD45. (Scale bar = 20 μm for CD29, CD54, and CD73 and 10 μm for CD90, CD105, CD34, CD14, and CD45).

Article Snippet: The following primary and secondary fluorescent antibodies were used for this characterization study: mouse anti-rat CD54-FITC conjugated antibody (1: 50 dilution, BD Pharmingen), monoclonal mouse anti-rat CD90-FITC conjugated antibody (1: 100 dilution, Millipore), mouse monoclonal IgG1 anti-CD34-FITC conjugated antibody (1: 100 dilution, Santa Cruz Biotechnology), mouse monoclonal anti-rat CD45-PE conjugated antibody (1: 100 dilution, BD Pharmingen), rabbit polyclonal IgG anti-CD14 antibody (1: 50 dilution, Santa Cruz biotechnology), monoclonal mouse anti-β 1 integrin antibody (1: 50 dilution, Millipore), monoclonal mouse anti-rat CD73 antibody (1: 50 dilution, BD Pharmingen), goat polyclonal IgG anti-CD105 antibody (1: 25 dilution, Santa Cruz Biotechnology), mouse monoclonal anti-MAP2 IgG1 antibody (1: 100 dilution, Millipore), mouse monoclonal anti-NeuN IgG1 antibody (1: 50 dilution, Millipore), and mouse monoclonal anti-Neurofilament IgG1 antibody (1: 100 dilution, Millipore), with appropriate secondary antibodies like goat anti-rabbit IgG-RPE antibody (1: 50 dilution, Jackson ImmunoResearch), goat anti-mouse IgG2b-RPE antibody (1: 50 dilution, Southern Biotech), goat anti-mouse IgG1-PE conjugated antibody (1: 50 dilution, Southern Biotech), donkey anti-goat IgG-perCP conjugated antibody (1: 100 dilution, Jackson ImmunoResearch), and goat anti-mouse IgG1-FITC antibody (1: 50 dilution, Southern Biotech).

Techniques: Immunohistochemical staining

Journal: Annals of Neurosciences

Article Title: Motor Recovery after Transplantation of Bone Marrow Mesenchymal Stem Cells in Rat Models of Spinal Cord Injury

doi: 10.1159/000487069

Figure Lengend Snippet: Surface marker expression of rat MSCs. Flow cytometry analysis of the immunophenotypic surface profiles for CD29, CD54, CD90, CD73, CD105, CD34, CD14, and CD45 of cultured MSCs. Histograms represent the counts of cells incubated with the relevant antibody. The logarithm on the x-axis represents the intensity of the fluorescent signal and the number of cells on the y-axis. Second passage cultured MSCs were positive for the markers CD29, CD54, CD73, CD90, and CD105 but negative for CD34, CD14, and CD45.

Article Snippet: The following primary and secondary fluorescent antibodies were used for this characterization study: mouse anti-rat CD54-FITC conjugated antibody (1: 50 dilution, BD Pharmingen), monoclonal mouse anti-rat CD90-FITC conjugated antibody (1: 100 dilution, Millipore), mouse monoclonal IgG1 anti-CD34-FITC conjugated antibody (1: 100 dilution, Santa Cruz Biotechnology), mouse monoclonal anti-rat CD45-PE conjugated antibody (1: 100 dilution, BD Pharmingen), rabbit polyclonal IgG anti-CD14 antibody (1: 50 dilution, Santa Cruz biotechnology), monoclonal mouse anti-β 1 integrin antibody (1: 50 dilution, Millipore), monoclonal mouse anti-rat CD73 antibody (1: 50 dilution, BD Pharmingen), goat polyclonal IgG anti-CD105 antibody (1: 25 dilution, Santa Cruz Biotechnology), mouse monoclonal anti-MAP2 IgG1 antibody (1: 100 dilution, Millipore), mouse monoclonal anti-NeuN IgG1 antibody (1: 50 dilution, Millipore), and mouse monoclonal anti-Neurofilament IgG1 antibody (1: 100 dilution, Millipore), with appropriate secondary antibodies like goat anti-rabbit IgG-RPE antibody (1: 50 dilution, Jackson ImmunoResearch), goat anti-mouse IgG2b-RPE antibody (1: 50 dilution, Southern Biotech), goat anti-mouse IgG1-PE conjugated antibody (1: 50 dilution, Southern Biotech), donkey anti-goat IgG-perCP conjugated antibody (1: 100 dilution, Jackson ImmunoResearch), and goat anti-mouse IgG1-FITC antibody (1: 50 dilution, Southern Biotech).

Techniques: Marker, Expressing, Flow Cytometry, Cell Culture, Incubation